PrimeTime qPCR Probes

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Items:

Sequence (5' → 3')

# Bases:

GC: % Tm: ºC DeltaG: kcal/mol

The optimal Tm for most probes is 5-10°C higher than their associated primers. If your calculated Tm is too low, check out IDT's list of Tm enhancing mods, such as Affinity Plus.

Notes

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The following items have errors:

Parameter sets


Target type
Oligo Conc	µM
Na⁺ Conc	mM
Mg⁺⁺ Conc	mM
dNTPs Conc	mM

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