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PrimeTime™ qPCR Primer Assays

Premixed primer pairs for gene expression analysis using intercalating dyes.

PrimeTime qPCR Primer Assays provide a primer pair designed for real-time PCR using intercalating dyes, such as SYBR® Green (Thermo Fisher Scientific), EvaGreen® (Biotium) dyes, as well as others. Predesigned sequences for human, mouse, or rat are designed with advanced bioinformatic and thermodynamic sequence analytics for easy selection.

Ordering

  • Advanced bioinformatics delivers the design you need with considerations for exon location and number of transcripts identified
  • Test target sequence amplification prior to using more expensive probe-based assays
  • Receive primer sequences with all orders

Forward and reverse primers, mixed and delivered in a single tube or well of a 96-well plate.

PrimeTime qPCR Primer Assays in tubes

Available in standard size, premixed, and shipped dry.


No. of Reactions (20 µL)PriceEstimated Ship DateQuantity (nmol)
PrimeTime qPCR Primers500$84.00 SGD2-3 business days5

1 Available for human, mouse, and rat targets (for assay configuration, choose intercalating dyes, primers only)

2 Choose design: qPCR–2 primers–intercalating dyes

PrimeTime qPCR Primer Assays in 96-well plates

Custom assays and predesigned qPCR sequences for human, mouse, and rat targets. Available in standard size, premixed, and shipped dry.


ProductReactions (20 µL)PriceEstimated Ship DateQuantity (nmol)
PrimeTime™ Primer Plate 500 $78.00 SGD 3 business days 5
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Create custom assays for any species with our free PrimerQuest™ Tool

Design custom sequences for your unique target using the PrimerQuest™ Tool—ideal for endpoint PCR, qPCR, and Sanger sequencing. Choose from optimized presets or customize parameters to fit your needs, all powered by the Primer3 engine.

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Product details

PrimeTime qPCR Primer Assays

  • The same primer pairs found in IDT’s PrimeTime qPCR 5' Nuclease Predesigned Assays
  • Compatible with SYBR® Green, EvaGreen®, and other intercalating dyes, where no probe is needed
  • Guarantee: Predesigned sequences will achieve 90% efficiency or better, or we will provide an alternative design free of charge. For more information, please contact us.

Predesigned PrimeTime qPCR Probe Assays are designed using IDT’s proprietary algorithm and optimized for melting temperature (Tm), single nucleotide polymorphism (SNP) avoidance (using NCBI RefSeq data), off-target amplification, splice variant recognition, and secondary structure predictions.

For commonly studied pathways in human, mouse, and rat species, we have suggested gene sets (see Resources section below) that can be used with our PrimeTime plate ordering system.

Transparency with every order

Orders include primer and probe sequences to help labs enhance publication credibility by adhering to MIQE guidelines, facilitate multiplex experiment design, assist with data interpretation and troubleshooting, and enable transcript validation.

Product data

PrimeTime qPCR Primer Assays yield high efficiency with intercalating dyes and probes

Figure 1. PrimeTime qPCR Primer Assays yield the same high efficiency whether used with intercalating dyes or probes. Amplification of 5 sequential 4-fold dilutions of cDNA using PrimeTime qPCR Primer Assays (with SYBR® Green dye [Bio-Rad SsoAdvanced Universal Probes Supermix]) or the PrimeTime qPCR 5′ Nuclease Assay (with dual-labeled probe [IDT PrimeTime Gene Expression Master Mix]) targeting human v-abl Abelson murine leukemia viral oncogene homolog 1 (ABL1, NM_005157.5).

Average reaction efficiency of 90% or more

Figure 2. PrimeTime qPCR Primer Assays have an average reaction efficiency >90%. Sixty-one randomly selected PrimeTime qPCR Primer Assays and 15 PrimeTime qPCR Primer Assays for endogenous control genes used with SsoAdvanced Universal Probes Supermix (Bio-Rad) were analyzed over 5 sequential 4-fold dilutions (50–0.195 ng/reaction) of cDNA prepared from qPCR Human Reference Total RNA (Agilent). Reactions were run on the CFX384 Touch Real-Time PCR Detection System (Bio-Rad) using PCR cycling conditions: 30 sec 95°C; 40 x (15 sec 95°C, 30 sec 60°C). Average reaction efficiencies for the assays tested exceeded 98%.

Resources

Gene sets for common pathways

Frequently asked questions

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