Please note that IDT Singapore will be closed on Monday, 11th August 2025 in observance of National Day in Lieu. For more information, please contact apac-custcare@idtdna.com.
The optimal Tm for most probes is 5-10°C higher than their associated primers. If your calculated Tm is too low, check out IDT's list of Tm enhancing mods, such as Affinity Plus.