Processing
SciTools™ Web Tools
These software applications determine the properties of any oligo sequence entered, as well as facilitate the intelligent design of assay conditions, all at the click of a button. The suite is easy to use and makes it simple to add each optimized oligonucleotide to an ongoing order list ready for purchase.
Oligo design & handling
- OligoAnalyzer™ Tool
Identifies oligonucleotide properties, including melting temperature, hairpins, dimers and mismatches
- UNAFold Tool
Identifies oligonucleotide properties, including melting temperature, hairpins, dimers and mismatches
- Dilution Calculator
Find the volume needed to dilute your oligo stock to a lower concentration
- Resuspension Calculator
Find the volume needed to resuspend a dry oligo to a desired concentration
qPCR assay design
- PrimeTime™ Predesigned qPCR sequences
Selects qPCR primers and probe sequences for human, mouse, and rat gene expression assays based on up-to-date sequence data
- PrimerQuest™ Tool
Allows highly customized designs for PCR primers and qPCR assays
- RealTime PCR Tool
Selects custom qPCR assays for any species
Genotyping
- rhAmp™ Genotyping Assay Design Tool
Choose predesigned rhAmp SNP Assays or design assays for SNPs not currently included in the predesigned assay library
CRISPR genome editing
- Alt-R™ Predesigned Cas9 crRNA Selection Tool
Selects guaranteed crRNAs targeting human, mouse, rat, zebrafish, or nematode genes
- Alt-R Custom Cas9 crRNA Design Tool
Designs custom crRNAs to sequences from any species
- Alt-R CRISPR HDR Design Tool
A complete solution for industry-leading HDR rates based on extensive wet bench testing and customer validation
- CRISPR-Cas9 crRNA design checker
Assesses on- and off-targeting potential of user-designed protospacer sequences
- rhAmpSeq™ CRISPR Analysis Tool
Flexible, cloud-based interrogation of CRISPR-mediated, double-strand break repair
Gene regulation & RNAi
- Predesigned DsiRNA Selection Tool
Selects DsiRNAs and TriFECTa® RNAi Kits for your human, rat, or mouse targets
- RNAi Design Tool
Generates DsiRNAs for any sequence from any species
Genes & gene fragments
- Codon Optimization Tool
Automates codon optimization of a gene to the species of your choice
- Genes & gene fragments order status
Track your order (Genes & gene fragments only)
Next generation sequencing
- Custom Adapter Configurator Tool
Guides you through choice of library prep method, configuration, indexing strategy, delivery format, and other options. Order directly online.
- Library Concentration Conversion Calculator
Please use the following format when citing SciTools:
PrimerQuest™ program, IDT, Coralville, Iowa, USA. Accessed 12 December, 2018. https://www.idtdna.com/SciTools.
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