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Features | Options |
---|---|
Design | Predesigned, custom, user-provided |
CRISPR systems | Cas9, Cas12a, Cas13, prime editing, and other alternative systems |
Guaranteed Yield | 0.5 nmol, 2 nmol, 5 nmol, and custom normalized deliverables |
Cas9 gRNA formats | 2-part cRNA:tracrRNA complex and sgRNA |
Custom lengths supported | 30-150 nt |
Chemical modifications | 2'-O-methyl RNA, PS linkages, end-blocking Alt-R modifications |
Plate types | 96- & 384-well PCR, Deep-well, V-bottom, ECHO, custom options available |
Formulation options | Multi-guide per well; pooled by gene Arrayed (single gRNA/well) Custom formulations upon request |
QC | Individual ESI/MS |
Supporting reagents & functional analysis pipeline (optional) | WT Cas9, HiFi Cas9, Cas12a, and Cas12a Ultra Glycerol-free options available in tubes or plates (ideal for robotics) Electroporation Enhancers rhAmpSeq™ CRISPR Analysis System (NGS-based on-/off-target editing analysis) |