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The Alt-R Cas9 nucleases and nickases are supplied at concentration of 10 mg/mL in 25 mM Tris-Cl, 300 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 50% glycerol (v/v), pH 7.4, with the exception of the Alt-R™ S.p. Cas9 V3, glycerol-free. The Alt-R™ S.p. Cas9 V3, glycerol-free is provided at a concentration of 10 mg/mL in a proprietary buffer without glycerol.