Frequently asked questions
Our team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.
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How do I design my CRISPR-Cas12a (Cpf1) crRNA guide sequence—correct orientation & strand?
Enter the 20–24 base DNA sequence downstream (3’ end) of the target Cas12a (Cpf1) PAM site (TTTV*), and then you are ready to continue. We typically recommend using a 21-nucleotide guide sequence.
The ordering tool will automatically convert the DNA sequence to RNA during the ordering process. If you are pasting your Cas12a (Cpf1) target site from an online design tool, make sure to verify the correct strand orientation before pasting the target sequence.
* V = A, C, or G